中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 82-86.doi: 10.3969/j.issn.1673-8225.2011. 01.018

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

小鼠移植物抗宿主病模型的建立

朱婕琳1，张  鹏1，陈  福2，侯如荣3   

  1. 厦门大学附属中山医院，1血液科，3放疗科，4检验科，福建省厦门市 361004；2厦门大学医学院动物实验室，福建省厦门市  361005
  • 收稿日期:2010-07-12 修回日期:2010-10-26 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 张鹏，硕士，主任医师，厦门大学附属中山医院血液科，361004 xm2981@163.com
  • 作者简介:朱婕琳★，女，1984年生，福建省厦门市人，汉族，厦门大学在读硕士，主要从事血液病学方面的研究。 zhujielin.happy@163.com
  • 基金资助:

    2009年福建省医学创新课题资助(2009-CXB-61)。课题名称：组蛋白去乙酰化酶抑制剂治疗移植物抗宿主病的实验研究。

Establishment of a mouse model of graft-versus-host disease

Zhu Jie-lin1, Zhang Peng1, Chen Fu2, Hou Ru-rong3   

  1. 1Department of Hematology, 3Department of Radiotherapy, 4Department of Laboratory, Zhongshan Hospital Affiliated to Xiamen University, Xiamen  361004, Fujian Province, China; 2Animal Laboratory, Medical College, Xiamen University, Xiamen  361005, Fujian Province, China
  • Received:2010-07-12 Revised:2010-10-26 Online:2011-01-01 Published:2011-01-01
  • Contact: Zheng Peng, Master, Chief physician, Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China xm2981@163.com
  • About author:Zhu Jie-lin★, Studying for master’s degree, Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China zhujielin.happy@163.com
  • Supported by:

    the Medical Innovation Project of Fujian Province in 2009, No. 2009-CXB-61

PDF

707

被引次数

8

摘要：

背景：小鼠移植物抗宿主病是异基因造血干细胞移植的主要并发症，表现为多系统损害(皮肤、食管、胃肠、肝脏等)的全身性疾病，是造成异基因造血干细胞移植后死亡的重要原因之一。
目的：探讨建立小鼠移植物抗宿主病模型的方法，为实验性研究药物防治移植物抗宿主病提供有效的动物模型。
方法：选择C57BL/6(H-2b)→BALB/C(H-2d)作为完全异基因移植供、受体。通过注射移植供鼠脾脏淋巴细胞及骨髓细胞数以建立移植物抗宿主病模型。根据临床表现、病理检查等判断移植物抗宿主病模型是否建立。
结果与结论：给予5×106个以上的供鼠脾细胞的小鼠都发生了急性移植物抗宿主病，但小鼠移植物抗宿主病出现的时间、存活期不同。提示，输入5×106个供鼠脾细胞可有效建立移植物抗宿主病模型，小鼠异基因骨髓移植动物模型是可靠的，可用于移植物抗宿主病防治的实验性研究。

关键词: 移植物抗宿主病, 小鼠, 动物模型, 移植, 异基因骨髓, 造血干细胞

Abstract:

BACKGROUND: Mouse graft-versus-host disease (GVHD) is the main complication of allogenic hematopoietic stem cell transplantation, presents the damages to multisystem (skin, esophago, stomach intestine, liver), and is one of reasons for death following allogenic hematopoietic stem cell transplantation.
OBJECTIVE: To explore the establishment of the mouse model of GVHD, and to provide effective animal models for experimental GVHD research.
METHODS: C57BL/6(H-2b)→BALB/C(H-2d) was selected as donor and recipient of complete allotransplantation. The GVHD models were established by injection of lymphocytes and bone marrow cells of the spleen of donor mouse. Whether GVHD models were established was assessed according to clinical situation and pathological examination.
RESULTS AND CONCLUSION: The recipient mice which accepted above 5×106 donor spleen cells developed acute GVHD after transplantation. However, the time when clinical situation of GVHD appeared and life span were different. Results indicated that the transfusion of 5×106 spleen cells was adequate to establish the mouse model of GVHD. The mouse model is reliable to the experimental research of GVHD.

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